Purification of Foot and Mouth Disease Virus Nonstructural Protein 3ABC from Vaccine in-process Samples and their Characterization

Order of Publishing in Issue: 
3
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
334-341
Authors: 
Anil Kumar Jangam[1], Sridevi V Nimmagadda[1] , Premalatha Dasari[†] and Rajendra Lingala[1]*
Address: 
[1]Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad- 500032, Telangana, India.
Address: 
[†]Department of Biotechnology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad- 500085, Telangana, India.

Foot and mouth disease is a highly contagious disease that affects the cloven hoofed animals which includes livestock and can impact on nation’s economy. Adjuvanted inactivated whole virus (140s) is used as vaccine to protect animals and control the disease. The vaccine production process is well established by many commercial manufacturers by culturing the virus on BHK cell line.

Keywords: 
FMDV, NSP, Chromatography, ELISA.

Development of Murine Monoclonal Antibodies, Characterization and Quantification of Diphtheria Toxoid in Vaccine Batches

Order of Publishing in Issue: 
2
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
318-333
Authors: 
Praveen Alagangula[1,2], Sridevi V Nimmagadda[1], Vidyasagar Pitta[1], Dipankar Das, Ralla Kumar[1], Shukra M Aavula[1], Mohammed Furman Ali[1], Premalatha Dasari[1], Rajendra Lingala[1]*
Address: 
[1]Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad- 500032, Telangana, India.
Address: 
[2]Department of Biotechnology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad- 500085, Telangana, India.

Diphtheria is caused by Cornybacteria diphtheria and it is one of the contagious diseases with a mortality rate of 5% to 10% worldwide. Though the mass immunization of diphtheria vaccine reduces the mortality, but the immediate effective treatment for this disease involves the administration of anti-diphtheria polyclonal antibodies or diphtheria antitoxin (DAT) produced from Equines. However the availability of antidiphtheria polyclonal antibody is very limited due to the less number of manufacturers.

Keywords: 
Monoclonal antibodies, Diphtheria toxin, ELISA, In vitro neutralization.

Compatibility and Stability of Ceftriaxone Sodium with Peritoneal Dialysis Solution

Order of Publishing in Issue: 
1
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
311-317
Authors: 
Osama Javed, Khalid Hussain, Muhammad Shahzad Aslam*
Address: 
University College of Pharmacy, University of the Punjab, Lahore, Pakistan
Address: 
*Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ziauddin University, Karachi, Pakistan

Drug compatibility and stability with the diluents is important expected therapeutic outcome. The administration of incompatible and unstable antibiotics admixtures is associated with several life threatening clinical situations. For the treatment of peritonitis or peritoneal infection, antibiotics are delivered at the site through peritoneal dialysis solution. Therefore, the investigation of compatibility and stability of antibiotic with peritoneal dialysis is imperative.

Keywords: 
Nil

NEWS ITEM

Order of Publishing in Issue: 
11
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
i-vi
Authors: 
Editor
Email-ID: 
editor@abap.co.in

News Item

Keywords: 
Nil

The Emergence of Blockchain Technology and its Impact in Biotechnology, Pharmacy and Life Sciences

Order of Publishing in Issue: 
10
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
304-310
Authors: 
L. N. Chavali[1], N. L. Prashanti[2], K. Sujatha[3] G. Rajasheker[1] and P.B. Kavi Kishor[1]
Address: 
[1]Department of Genetics, Osmania University, Hyderabad 500 007, India
Address: 
[2]GITAM Institute of Management, GITAM University, Rushikonda, Visakhapatnam 530 045, Andhra Pradesh, India
Address: 
[3]Department of Chemistry, KTR Women’s College, Gudivada 521 301, Andhra Pradesh, India
Email-ID: 
pbkavi@yahoo.com

The emergence of blockchain technology is regarded as 4th industrial revolution i.e., the integration of cyber-physical systems since the advent of Internet with far reaching applications in Banking, Insurance and Government, may impact other sectors especially life sciences. The fourth revolution is characterized by fusion of technologies that is blurring the lines between the physical, digital, and biological spheres.

Keywords: 
Blockchain, Agriculture, Pharmacy, Genomecoin, Cryptography, Smart contract.

Potential health risk assessment of heavy metal accumulation in the selected food fishes from Krishna Esturine region of Southern Deltaic Region of India

Order of Publishing in Issue: 
9
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
295-303
Authors: 
P.V Krishna* K. Prabhavathi and R.Prakasa Rao
Address: 
Department of Zoology & Aquaculture, Acharya Nagarjuna University, Nagarjuna Nagar – 522 510, Andhra Pradesh., India.
Email-ID: 
drpvkrishna@gmail.com

Rapid development of industries result in the production of high amounts of solid or liquid wastes in the form of effluents, which are usually discarded into the nearby water bodies causing contamination of ecosystem. These industrial effluents released can pose great health hazards to aquatic organisms particularly fishes.

Keywords: 
Heavy metals, Krishna estuarine region, Mugil cephalus, Sillago sihama.

Phytochemical analysis and in vitro antimicrobial activities of Terminalia arjuna leaf, bark and fruit extracts in different solvents

Order of Publishing in Issue: 
8
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
286-294
Authors: 
Vishwajeet Singh[1], Alok Bharadwaj[2], Nitin Wahi[2]* and Vijay Laxmi Tripathi[3]
Address: 
[1]Department of Botany, R.B.S College, Dr. B.R. Ambedkar University, Agra-282 002, U.P., India
Address: 
[2]Department of Biotechnology, GLA University, Mathura-281 406, U.P., India
Address: 
[3]College of Biotechnology, DUVASU, Mathura-281 001, U.P., India
Email-ID: 
wahink@gmail.com

The soxhlet extracts of fruits, bark, and leaf of Terminalia arjuna were obtained using different solvent viz. water, methanol, ethanol, acetone, chloroform and petroleum ether and were analyzed for their phytochemical, antibacterial and antifungal activity. The phytochemical activity of leaf, bark and fruit extract of T. arjuna were performed using all six solvent. Results clearly indicate the presence of alkaloids, carbohydrates, cardiac glycosides, proteins, phytosterols, flavonoids, tannins, terpenoids, saponins, and phenols/ polyphenols.

Keywords: 
Terminalia arjuna, Bark, Leaves, Fruit, Phytochemical Screening.

Characterization and Evaluation of Antibacterial, Antioxidant and Cytotoxicity of synthesised silver nanoparticles (AgNps) using chloroform crude callus extracts of Wrightia tinctoria (Roxb.)

Order of Publishing in Issue: 
7
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
275-285
Authors: 
Keerthi Priya Yadala and Asha S *
Address: 
Department of Biotechnology, VFSTRU, Vadlamudi, Guntur Dist., A.P
Email-ID: 
sai848@gmail.com

The present work established that the chloroform callus extract taken from the leaf midrib of in vitro grown seedling of Wrightia tinctoria (Roxb.) is very efficient for the synthesis of AgNPs. The produced silver nanoparticles were preliminarily conformed by UV Visible spectroscopy, SEM, TEM, FTIR spectroscopy and Energy Dispersion Spectrum. UV-Vis spectroscopy analysis revealed the peak at 425nm which corresponds to the surface plasmon resonance of AgNPs. The uniform spherical shape and size was detected by SEM and TEM.

Keywords: 
WTR leaf midrib callus, Silver nanoparticles, Bio-reductant, DPPH inhibition, Antibacterial activity, Antioxidant and Cytotoxicity.

Optimization of a simple methodology for Extraction, purification of SDG from Linumusitatissimumand its characterization

Order of Publishing in Issue: 
6
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
267-274
Authors: 
Guna Bhushana Daddala[a]*, A. Swaroopa Rani[b] and Srinivas Papineni[c]
Address: 
[a]Department of Biotechnology, Research Scholar (JNTUA), Jawaharlal Nehru Technological University, Ananthapur,515002, India
Address: 
[b]Department of Biotechnology, Asst. professor (JNTUA), Department of Biotechnology, Jawaharlal Nehru Technological University, Ananthapur, 515002, India
Address: 
[c]JodasexpoimPvt Ltd, 1359, Road Number 45, Jubilee Hills, Hyderabad, 500033, India

This research was conducted to optimize a simple methodology to extract and purify a lignan present in Linumusitatissimum (Flax seed also called as line seed). Flax seeds are the richest plant source of lignans and omega-3 fatty acid. SDG - Secoisolariciresinol Diglucoside is the principallignan present in flax seeds.The extraction and purification methods vary from simple to complex depending on separation, fractionation, identification and detection of the analytes.

Keywords: 
Flaxseed, lignan, secoisolariciresinol diglucoside, TLC, IR, LCMS, NMR.

Fingerprint Profile of an important therapeutic plant of Astavarga Crepidium acuminatum (D. Don) Szlach by HPTLC

Order of Publishing in Issue: 
5
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
257-264
Authors: 
Mamta Arora*[1], Anupama Mahajan[2], Jaspreet K. Sembi[3]
Address: 
[1]Research Scholar of IKG, PTU Jallandhar, Faculty of Biotechnology, ASBASJSM College, Bela Rupnagar, Punjab
Address: 
[2]Assistant Professor in Biotechnology, SUSCET, Tangori, Mohali, Punjab
Address: 
[3]Assistant Professor in Botany, Panjab University, Chandigarh

Nature is crucial source of drugs. Plants have played important role in identifying important drugs and are basis of various modern pharmaceuticals. Therefore ethnomedicinal plants must be exploited to identify lead compounds. C. acuminatum is an important plant of Astavarga (combination of 8 drugs, most commonly used in Ayurveda) C. acuminatum is used in breathing disorders, burning sensation, cough, decrease in bone tissue, bleeding disorders, blood disorders, tuberculosis, insect bites and rheumatism.

Keywords: 
HPTLC, Fingerprint profile, C. acuminatum (Jeevak), Malaxis acuminata Astavarga, Phytochemical analysis.

 
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