Development of Monoclonal Antibodies Against Cry1Ac/ Ab Protein for Designing of Sandwich ELISA to Detect BT Toxin from Cotton Seeds and Leaves

Order of Publishing in Issue: 
2
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
218-229
Authors: 
Seetharavamma Gnagadasu[1&2], Jayant K Bhanushali[1], Usha Kiranmayi Mangamuri[3], K. R. S. Sambasiva Rao[1], Sudhakar Poda[1*]
Address: 
[1]Amar Immunodiagnostics Pvt, Ltd, Plot No 242/1, Road No 18, Jubilee Hills, Hyderabad-500033, Telangana, India
Address: 
[2]Department of Botany and Microbiology and Department of Biotechnology, Acharya Nagarjuna University, Guntur-522510, Andhra Pradesh, India
Address: 
[3]Department of Biotechnology, Acharya Nagarjuna University, Guntur-522510, Andhra Pradesh, India

The design of the study is to develop monoclonal antibodies against Cry1Ac/Ab protein for designing os sandwich ELISA (hybridoma technology). Hybridoma technology was invented by Cesar Milstein and Georges J.F Kohler in the year 1975 and is an unique method used to produce identical antibodies in maximum quantities. Monoclonal antibodies were developed by immunization of Balb/C mice with Cry1Ac/Ab Protein. Titer values of mice tail bleeds were checked and the best mice with higher titer value was used for fusion.

Keywords: 
Bacillus thuringiensis, Hybridoma Technology, Cry1Ac/Ab, HAT medium, Monoclonal antibodies, Iso-typing, genetically modified crops and Immunization.

Chart Review Study of Subjects Administered Amnionic Membrane for Treatment of Joint Pain

Order of Publishing in Issue: 
1
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
212-217
Authors: 
Ruben Berrocal Timmons[1]*, Neal E. Fearnot[2] and Michael E. Leckrone[2]
Address: 
[1]Interventional Rehabilitation Center, Pensacola, Florida, USA
Address: 
[2]Cook Advanced Technologies, West Lafayette, Indiana, USA
Email-ID: 
rbtmd1@gmail.com

Context: Treatment of joint pain with injection of amnionic membrane has not been adequately studied. Objectives: Determine if patients who received cryopreserved particulate amnionic membrane (CPAM) injected into painful back and knee joints experience less back or knee pain, improve physical ability, and use less opiates and NSAIDs over a 12 week time period.

Keywords: 
Amnion, Amnionic membrane, Joint pain, Knee pain, Back pain.

News Item

Order of Publishing in Issue: 
11
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
i - vi
Authors: 
Editor
Email-ID: 
editor@abap.co.in

News Items

Keywords: 
News item

From Natural products to therapeutically important antifungals

Order of Publishing in Issue: 
10
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
206-212
Authors: 
Neelabh and Karuna Singh
Address: 
Department of Zoology (MMV), Banaras Hindu University Varanasi -221005, India

Although, there has been a significant progress in the field of pharmaceuticals and drug designing in the recent past, yet there is a vacuum in the development of broad spectrum antifungal drugs. Those drugs that are at our disposal are not free from side effects having several risks and associated toxicities on prolonged usage. Therefore, there has been an ever escalating demand of devising new drugs that are preferably natural and free from side effects.

Keywords: 
Natural, Antifungal, Diseases, Chitin synthase inhibitors.

Targeting Cancer cell metabolism via Target of Rapamycin

Order of Publishing in Issue: 
9
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
196-205
Authors: 
Ankita Awasthi[1], Vikrant Nain[1], Himanshi Singh[1] , Pavan Kumar[1], Rekha Puria[1]*
Address: 
[1]School of Biotechnology, Gautam Buddha University, Greater NOIDA, Gautam Budh Nagar-201312, India
Email-ID: 
rpuria@gbu.ac.in

Cancer cells acquire many metabolic rearrangements to provide energy and macromolecules required for continuous growth and proliferation. Warburg suggested that cancerous cells rely only on glycolysis for energy and biosynthesis of macromolecules. It’s still unexplainable that how this metabolic switch allows predominance of cancer cell in the hypoxic and metabolically highly active conditions around the cancerous cells.

Keywords: 
Nil

Molecular Taxonomy of Associated Microbes From Sea Slug Kalinga Ornata and its bioactivity

Order of Publishing in Issue: 
8
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
187-195
Authors: 
M. Mohanraj[1], N. Sri Kumaran[1,2]* and S. Bragadeeeswaran[1]
Address: 
[1]Faculty of Marine Science, Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai – 608 502, Tamil Nadu, India
Address: 
[2]Department of Marine Biotechnology, AMET University, Kanathur, Chennai 603112, Tamil Nadu, India
Email-ID: 
s.kumaran08@gmail.com

This study was carried to investigate the molecular taxonomy of associated microbes from sea slug Kalinga ornata and its bioactivity form Indian coastal waters. In this study, totally 27 associated microbes isolated from sea slug K. ornate. Morphologically 5 different isolates were selected for sequencing and submitted to NCBI. Further these 5 sequences were analysed for the phylogenetic relationship and percentage of the nucleotide contents with few isolates sequences extracted via FASTA format from NCBI.

Keywords: 
Sea slug, Molecular taxonomy, Sequencing, Antagonistic, Heamolytic.

System Modeling of AkT using Linear and Robust Regression Analysis

Order of Publishing in Issue: 
7
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
177-186
Authors: 
Shruti Jain
Address: 
Department of Electronics and Communication Engineering, Jaypee University of Information Technology, Himachal Pradesh

This paper presents the review of different types of modeling like deterministic and probabilistic. Main stress of this paper is on Probabilistic modeling. Probabilistic modeling is further divided into three types : predictive, regression and correlation modeling. The analysis was done using three different input proteins and four different output proteins. A model was made using different analysis techniques which presents the r2 values for one of the marker protein called AkT.

Keywords: 
AkT, Linear Regression Analysis, Robust Regression Analysis, Partial Least Square.

In-Vivo Evaluation of Rifampicin Loaded Nanospheres: Biodistribution and Mycobacterium Screening Studies

Order of Publishing in Issue: 
6
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
169-176
Authors: 
Vishnu Vardhan Reddy Beeram[1], Krupanidhi S[1]*& Venkata Nadh R[2]
Address: 
[1]Department of Biotechnology, Vignan’s University, Vadlamudi, Guntur-522213, India.
Address: 
[2]GITAM University – Bengaluru Campus, Karnataka- 561 203, India.

Rifampicin PLGA nanospheres are formulated with a specific goal in order to decrease the dose, adverse effects and to enhance targeted drug delivery. Rifampicin nanospheres were prepared and evaluated by emulsion solvent evaporation method. In vivo bio distribution studies reveal that there was a long term accumulation of rifampicin nanospheres in the lungs over other organs. The increase in Cmax values confirmed that inhalable PLGA nanospheres are suitable for targeting and providing sustained release of antitubercular drugs to lungs.

Keywords: 
Rifampicin Nanospheres, M. tuberculosis, In vivo Bio distribution studies.

Optimization of process parameters for Poly Hydroxy Butyrate Production from Isolated Acinetobacter nosocomialis RR20 through Submerged Fermentation

Order of Publishing in Issue: 
5
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
159-168
Authors: 
A. Ranganadha Reddy[a], T.C. Venkateswarulu[a]*, P. Sudhakar[c], S. Krupanidhi[a], K. Vidya Prabhakar[b]*
Address: 
[a & a*]Department of Biotechnology Vignan’s Foundation for Science, Technology & Research, Valdamudi-522 213, India
Address: 
[b]*Department of Biotechnology Vikrama Simhapuri University SPSR Nellore-524003, India
Address: 
[c]Department of Biotechnology Acharya Nagarjuna University Guntur-522510, India

Poly Hydroxy Butyrate member of polyhydroxyalkanoates family and is generally used as an alternative to polypropylene based plastic. Production of Poly Hydroxy Butyrate, biodegradable polymer from industrial wastes has several advantages such as recycle of waste and the production of high valuable products. It has been isolated from various sources till date. In this study, different strains of isolated bacteria were evaluated for their PHB productivity, but Acintobacter nosocomialis RR20 strain resulted highest production.

Keywords: 
PHB, A.nosocomialis RR20, Shake flask culture, Process variables.

Spectroscopic studies, Antioxidant and Anticancer attributes of diffusible eumelanin produced by marine Streptomyces rochei

Order of Publishing in Issue: 
4
Volume :12
Issue :2
April, 2018 - June, 2018
Page No: 
147-158
Authors: 
Satheesh Periyasamy[1], Shalini Devi.S[2], Preethi Kathirvel[1]
Address: 
[1]Department of Microbial Biotechnology, Bharathiar University, Coimbatore, India.
Address: 
[2]Department of microbiology, Bhavans Vivekananda college, Hyderabad, India

The diffusible melanin pigment from marine Streptomyces rochei isolated from Vishakhapatnam coast was characterized using UV Spectrophotometer, GC-MS, NMR, EPR Spectroscopy, CHNS elemental analysis, Ramon Spectroscopy and found to be a pure form of eumelanin.

Keywords: 
Eumelanin, Spectroscopy, MTT, DPPH, COMET.

 
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