Determination of Enantiomeric Purity of Esomeprazole Pharmaceutical Products using Validated HPLC Method

Order of Publishing in Issue: 
2
Volume :13
Issue :1
January, 2019 - March, 2019
Page No: 
20-25
Authors: 
Bashar Al-Sabti*, Mohamed Ammar Al Khayat,Mohamed Aamer Al-Mardini
Address: 
Department of Drug Quality Control and Pharmaceutical Chemistry, Faculty of Pharmacy, University of Damascus, Syria

This method was developed to perform a comparative study of enantiomeric purity of some products of esomeprazole (capsules, tablets) manufactured locally and in some neighboring countries for the determination the percentage of R enantiomer. A product of under license company was used for comparison of the results. The study was performed by using a validated HPLC method on chiral column Nucleocel Alpha S and a mixture ethanol: hexane (70:30, v/v) as a mobile phase, the flow rate was 0.65 ml/min, and the detection was carried out using UV detector at 302nm.

Keywords: 
Enantiomeric Purity, Omeprazole, Esomeprazole, HPLC.

Generation of FMDV Recombinant 3ABC Protein and Development of Monoclonal Antibodies as Reagents for 3ABC Quantitative ELISA

Order of Publishing in Issue: 
1
Volume :13
Issue :1
January, 2019 - March, 2019
Page No: 
07-19
Authors: 
Anil Kumar Jangam[1,2], Sridevi V Nimmagadda[1], Madan Mohan M, Ravi R Varma, Shukra Madhaha A[1], Praveen Alagangula[1], Premalatha Dasari[2], Lingala Rajendra[1]*
Address: 
[1]Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad- 500032, Telangana, India
Address: 
[2]Department of Biotechnology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad- 500085, Telangana, India

FMDV is one of the most contagious disease in cloven hoofed animals and it is known to cause lameness, vesicular lesions on tongue, feet, teats and snout with reduced milk production, loss of weight and high mortality in young animals. Many countries being endemic, continuous vaccination might help in attaining disease free status.

Keywords: 
Foot and mouth disease, Monoclonal antibody, Vaccine, ELISA, Non-structural proteins.

News Item

Order of Publishing in Issue: 
11
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
i-vii
Authors: 
Editor
Email-ID: 
editor@abap.co.in

News Item

Keywords: 
Nil

Modelling and Optimization of L-Asparaginase Production from Bacillus stratosphericus

Order of Publishing in Issue: 
10
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
390-405
Authors: 
Madhuri Pola[1], Chandrasai Potla Durthi[1], Satish Babu Rajulapati[1]*, Rajeswara Reddy Erva[2]
Address: 
[1]National Institute of Technology, Warangal
Address: 
[2]National Institute of Technology, Andhra Pradesh

L-Asparaginase has gained importance as one of the most important medications used to treat Acute Lymphocytic Leukemia (ALL). The goal of the current study is to maximize the LAsparaginase activity besides minimizing the LGlutaminase activity. Shorter half-life and side effects remain the drawbacks of the commercially available L-Asparaginase drugs. To overcome the drawbacks, L-Asparaginase has been isolated from endophytes of Ocimum tenuiflorum.

Keywords: 
L-Asparaginase, ALL, endophytes, OFAT, Plackett-Burman method, RSM.

Evaluation of Factors Affecting L-asparaginase Activity using Experimental Design

Order of Publishing in Issue: 
9
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
377-389
Authors: 
Rania A. Zaki, Mona S. Shafei, Heba A. El Refai *, Abeer A. El-Hadi and Hanan Mostafa
Address: 
Chemistry of Natural and Microbial Products Department, National Research Center, (ID:60014618), Dokki, Giza, Egypt
Email-ID: 
dr.heba_ar@yahoo.com

The present study aims for the purification and characterization of L-asparaginase produced by Fusarium solani. Microbial L-Asparaginase has attracted considerable attention, owing to the cost effective and eco-friendly nature. The most common use of asparaginases is as a processing aid in the manufacture of food. L-asparaginase used to reduce the formation of acrylamide, a suspected carcinogen, in starchy food products such as baked or fried snacks and biscuits.

Keywords: 
Fusarium solani -L-Asparaginase - Solid State Fermentation- partially purified enzyme- sequential statistical strategy.

Molecular Phylogenetic Analysis of Indian Apple Snail

Order of Publishing in Issue: 
8
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
371-376
Authors: 
Silpi Sarkar and S. Krupanidhi
Address: 
Department of Biotechnology, VFSTR Vadlamudi, 522213 A P, India

Apple snails (Ampullariidae) belong to a diversified freshwater family which occurs in pantropical habitats. The Indian apple snail, Pila globosa and other operculate snails are grouped in Architaenioglossa which also includes Viviparidae and Cyclophoridae. In our study, a concise relationship of Ampullariidae with Viviparidae and Cyclophoridae has been elucidated by using partial nucleotide sequence of cytochrome b gene.

Keywords: 
Pila globosa, Morphometry, Cytochrome b gene, RAxML , MEGA-v5.2

Protein Characterization at Atomic Level: A Novel Approach for Sequence Analysis

Order of Publishing in Issue: 
7
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
367-370
Authors: 
Parul Johri[1]*, Mala Trivedi[1], Drishti Srivastava[1], Aman Kumar Singh[1] and Mohammed Haris Siddiqui[2]
Address: 
[1]Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Malhaur, Gomti Nagar Extension, Lucknow, Uttar Pradesh, India
Address: 
[2]Department of Bioengineering, Integral University Lucknow, Uttar Pradesh, India
Email-ID: 
pjohri@lko.amity.edu

Carbon being the most omnipresent element in all the organic compounds is of great importance with regards to its structure and function. The fundamental structure of protein is composed of amino acids arranged in linear chain and folded to a globular form. All twenty amino acids consist of combination of only five different atoms that are Carbon, Nitrogen, Oxygen, Hydrogen and Sulphur. Depending on the property of their side chain the amino acids are classified as hydrophobic and hydrophilic.

Keywords: 
Carbon, E. Coli, dynamic programming, proteins.

Evaluation of Antimicrobial Activity of Emblica officinalis against Skin Associated Microbial Strains

Order of Publishing in Issue: 
6
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
355-366
Authors: 
Lovey Sharma[1]* and Ram Kumar Pundir[1]
Address: 
[1]Department of Biotechnology, Ambala College of Engineering and Applied Research, Devsthali (near Mithapur), P.O. Sambhalkha-133101, Ambala (Haryana)-India

The present study was undertaken to access antimicrobial activity of fruit extracts of Amla (Emblica officinalis) against skin associated microorganisms. The antimicrobial activity of five different solvents viz. methanol, ethanol, distilled water, chloroform and petroleum ether against Gram positive, Gram negative bacteria and yeast namely Propionibacterium acne, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans was assessed by using agar well diffusion method.

Keywords: 
Antimicrobial activity, Emblica officinalis fruit, GC-MS analysis, Phytochemicals, Skin disease.

Insilico Studies of FOR20 - A Centrosomal Protein

Order of Publishing in Issue: 
5
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
349-354
Authors: 
A. RanganadhaReddy[1]*, N. MadhanSai[1], S. Krupanidhi[1], P. Sudhakar[2], and T.C. Venkateswarulu[1]
Address: 
[1*]Department of Biotechnology, VFSTR (Deemed to be University) Guntur - 522213, India
Address: 
[2]Department of Biotechnology, Acharya Nagarjuna University Guntur - 522 510, India
Email-ID: 
rangaaluri@gmail.com

There is enough scientific evidence on microtubule playing important role in various functions, one such is chromosomal segregation, which made them as primary targets in the cancer treatment. Centrosomal proteins plays vital role in maintaining microtubule dynamics that having significant effect on various functions involving microtubule such as chromosomal segregation, intracellular transport and cell polarity.

Keywords: 
FOR20 protein, Centrosomal protein, SWISS MODEL, PROCHECK.

An in silico Structural study on Bacterial Sulfite Reductase

Order of Publishing in Issue: 
4
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
342-348
Authors: 
Rajeswara Reddy Erva[1]*, Rinku.P.Varghese[2], A.L.Prasanna[2], G.Madhunika[2], K.S.Ravi Teja[2], V.S.Santosh[2], Satish Babu Rajulapati[3]
Address: 
[1]Department of Biotechnology, National Institute of Technology Andhra Pradesh, Tadepalligudem, Andhra Pradesh, India.
Address: 
[2]School of Biotechnology, VFSTR (Deemed to be University), Vadlamudi, Andhra Pradesh, India.
Address: 
[3]Department of Biotechnology, National Institute of Technology Warangal, Warangal, Telangana, India.

Sulfur is amongst the most profuse elements on the globe and a vital component for life maintenance. Sulfite reductase plays a key role in sulfur pathway. It converts sulfite to sulfide. Reductase enzyme is present in sulfur reducing bacteria. Three organisms from Desulfovibrio species namely, D. alaskensis, D. vulgaris, Desulfovibrionaceae are used in this study. The computational analysis has been done out on the sulfite reductase enzyme form the above listed sulfur reducing bacteria using in silico tools.

Keywords: 
Sulfite Reductase, Sulfur reducing bacteria, Structure analysis, Homology modeling and structural validation.

 
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