NEWS ITEM

Order of Publishing in Issue: 
11
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
i-vi
Authors: 
Editor
Email-ID: 
editor@abap.co.in

News Item

Keywords: 
Nil

The Emergence of Blockchain Technology and its Impact in Biotechnology, Pharmacy and Life Sciences

Order of Publishing in Issue: 
10
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
304-310
Authors: 
L. N. Chavali[1], N. L. Prashanti[2], K. Sujatha[3] G. Rajasheker[1] and P.B. Kavi Kishor[1]
Address: 
[1]Department of Genetics, Osmania University, Hyderabad 500 007, India
Address: 
[2]GITAM Institute of Management, GITAM University, Rushikonda, Visakhapatnam 530 045, Andhra Pradesh, India
Address: 
[3]Department of Chemistry, KTR Women’s College, Gudivada 521 301, Andhra Pradesh, India
Email-ID: 
pbkavi@yahoo.com

The emergence of blockchain technology is regarded as 4th industrial revolution i.e., the integration of cyber-physical systems since the advent of Internet with far reaching applications in Banking, Insurance and Government, may impact other sectors especially life sciences. The fourth revolution is characterized by fusion of technologies that is blurring the lines between the physical, digital, and biological spheres.

Keywords: 
Blockchain, Agriculture, Pharmacy, Genomecoin, Cryptography, Smart contract.

Potential health risk assessment of heavy metal accumulation in the selected food fishes from Krishna Esturine region of Southern Deltaic Region of India

Order of Publishing in Issue: 
9
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
295-303
Authors: 
P.V Krishna* K. Prabhavathi and R.Prakasa Rao
Address: 
Department of Zoology & Aquaculture, Acharya Nagarjuna University, Nagarjuna Nagar – 522 510, Andhra Pradesh., India.
Email-ID: 
drpvkrishna@gmail.com

Rapid development of industries result in the production of high amounts of solid or liquid wastes in the form of effluents, which are usually discarded into the nearby water bodies causing contamination of ecosystem. These industrial effluents released can pose great health hazards to aquatic organisms particularly fishes.

Keywords: 
Heavy metals, Krishna estuarine region, Mugil cephalus, Sillago sihama.

Phytochemical analysis and in vitro antimicrobial activities of Terminalia arjuna leaf, bark and fruit extracts in different solvents

Order of Publishing in Issue: 
8
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
286-294
Authors: 
Vishwajeet Singh[1], Alok Bharadwaj[2], Nitin Wahi[2]* and Vijay Laxmi Tripathi[3]
Address: 
[1]Department of Botany, R.B.S College, Dr. B.R. Ambedkar University, Agra-282 002, U.P., India
Address: 
[2]Department of Biotechnology, GLA University, Mathura-281 406, U.P., India
Address: 
[3]College of Biotechnology, DUVASU, Mathura-281 001, U.P., India
Email-ID: 
wahink@gmail.com

The soxhlet extracts of fruits, bark, and leaf of Terminalia arjuna were obtained using different solvent viz. water, methanol, ethanol, acetone, chloroform and petroleum ether and were analyzed for their phytochemical, antibacterial and antifungal activity. The phytochemical activity of leaf, bark and fruit extract of T. arjuna were performed using all six solvent. Results clearly indicate the presence of alkaloids, carbohydrates, cardiac glycosides, proteins, phytosterols, flavonoids, tannins, terpenoids, saponins, and phenols/ polyphenols.

Keywords: 
Terminalia arjuna, Bark, Leaves, Fruit, Phytochemical Screening.

Characterization and Evaluation of Antibacterial, Antioxidant and Cytotoxicity of synthesised silver nanoparticles (AgNps) using chloroform crude callus extracts of Wrightia tinctoria (Roxb.)

Order of Publishing in Issue: 
7
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
275-285
Authors: 
Keerthi Priya Yadala and Asha S *
Address: 
Department of Biotechnology, VFSTRU, Vadlamudi, Guntur Dist., A.P
Email-ID: 
sai848@gmail.com

The present work established that the chloroform callus extract taken from the leaf midrib of in vitro grown seedling of Wrightia tinctoria (Roxb.) is very efficient for the synthesis of AgNPs. The produced silver nanoparticles were preliminarily conformed by UV Visible spectroscopy, SEM, TEM, FTIR spectroscopy and Energy Dispersion Spectrum. UV-Vis spectroscopy analysis revealed the peak at 425nm which corresponds to the surface plasmon resonance of AgNPs. The uniform spherical shape and size was detected by SEM and TEM.

Keywords: 
WTR leaf midrib callus, Silver nanoparticles, Bio-reductant, DPPH inhibition, Antibacterial activity, Antioxidant and Cytotoxicity.

Optimization of a simple methodology for Extraction, purification of SDG from Linumusitatissimumand its characterization

Order of Publishing in Issue: 
6
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
267-274
Authors: 
Guna Bhushana Daddala[a]*, A. Swaroopa Rani[b] and Srinivas Papineni[c]
Address: 
[a]Department of Biotechnology, Research Scholar (JNTUA), Jawaharlal Nehru Technological University, Ananthapur,515002, India
Address: 
[b]Department of Biotechnology, Asst. professor (JNTUA), Department of Biotechnology, Jawaharlal Nehru Technological University, Ananthapur, 515002, India
Address: 
[c]JodasexpoimPvt Ltd, 1359, Road Number 45, Jubilee Hills, Hyderabad, 500033, India

This research was conducted to optimize a simple methodology to extract and purify a lignan present in Linumusitatissimum (Flax seed also called as line seed). Flax seeds are the richest plant source of lignans and omega-3 fatty acid. SDG - Secoisolariciresinol Diglucoside is the principallignan present in flax seeds.The extraction and purification methods vary from simple to complex depending on separation, fractionation, identification and detection of the analytes.

Keywords: 
Flaxseed, lignan, secoisolariciresinol diglucoside, TLC, IR, LCMS, NMR.

Fingerprint Profile of an important therapeutic plant of Astavarga Crepidium acuminatum (D. Don) Szlach by HPTLC

Order of Publishing in Issue: 
5
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
257-264
Authors: 
Mamta Arora*[1], Anupama Mahajan[2], Jaspreet K. Sembi[3]
Address: 
[1]Research Scholar of IKG, PTU Jallandhar, Faculty of Biotechnology, ASBASJSM College, Bela Rupnagar, Punjab
Address: 
[2]Assistant Professor in Biotechnology, SUSCET, Tangori, Mohali, Punjab
Address: 
[3]Assistant Professor in Botany, Panjab University, Chandigarh

Nature is crucial source of drugs. Plants have played important role in identifying important drugs and are basis of various modern pharmaceuticals. Therefore ethnomedicinal plants must be exploited to identify lead compounds. C. acuminatum is an important plant of Astavarga (combination of 8 drugs, most commonly used in Ayurveda) C. acuminatum is used in breathing disorders, burning sensation, cough, decrease in bone tissue, bleeding disorders, blood disorders, tuberculosis, insect bites and rheumatism.

Keywords: 
HPTLC, Fingerprint profile, C. acuminatum (Jeevak), Malaxis acuminata Astavarga, Phytochemical analysis.

Identification of Thermophilic Flavobacterium and Anoxybacillus in Unexplored Tatapani Hot spring of Kishtwar District of Jammu and Kashmir: A North Western Himalayan State

Order of Publishing in Issue: 
4
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
245-256
Authors: 
Sonum Sharma[1], Parul Sharma[1], Anuradha Sourirajan[1] David J. Baumler[c,d,e] and Kamal Dev[1c*]
Address: 
[1]Faculty of Applied Sciences and Biotechnology, Shoolini University, Post Box No.9, Head Post Office, Solan (H.P.)-173212.
Address: 
[c]Department of Food Science and Nutrition, [d]Microbial and Plant Genomic Institute, and [e]Biotechnology Institute, University of Minnesota-Twin Cities St. Paul, Minnesota, USA

Two industrially important thermophilic bacteria were isolated from Tatapani hot spring located in Kishtwar district of Jammu and Kashmir, India and were named as KSI and KSII. Water sample from Tatapani hot spring was collected for screening of industrially important thermophillic bacteria. Only two thermophilic bacteria (KSI and KSII) were isolated from Tatapani hot spring water sample. The strict thermophilic nature of both the bacterial isolates (KSI and KSII) was tested by growing them at different temperatures ranging from 40°C-70°C.

Keywords: 
Kishtwar, Himalayas, Flavobacterium thermophilum, Anoxybacillus.

Molecular Characterization and optimization of Bioactive Compounds Production of three Actinomycetes spp Isolated from Waste Dump Soil from Western Uganda

Order of Publishing in Issue: 
3
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
230-244
Authors: 
Adamu Almustapha Aliero[1,2], Abubakar Sunusi Adam[1], Ibrahim Ntulume[1], Ahmad Ibrahim Bagudo[2], Aminu Ado B/Kudu[3], Miruka Conrad Ondieki[4], Sanusi Ahmad Jega[4], John Odda[3], Matilda Angela Okech[5]
Address: 
[1]Department of Microbiology and Immunology, Kampala International University Western campus, Uganda
Address: 
[2]Department Biological Sciences Kebbi State University of Science and Technology, Aliero, Nigeria
Address: 
[3]Department of Pharmacology, Kampala International University-Western Campus, Uganda
Address: 
[4]Department of Biochemistry, Kampala International University-Western Campus, Uganda
Address: 
[5]College of Health, Medicine and Life Sciences, Department of Microbiology, St. Augustine International University, Uganda

The study was designed to investigate the effect of growth culture conditions, namely: Media composition, incubation period, temperature and pH on the production of antimicrobial compounds from three selected actinomycetes isolated from waste dump soil in Western Uganda. Molecular characterizations of these selected isolates were also carried out. Optimization processes were assessed using shake-flask cultures on eight different media, Portion of filtered fermentation broths were assayed using agar well diffusion method.

Keywords: 
Optimization, Actinomycetes spp, Bioactive Compound, Molecular characterization, Waste dump soil, Western Uganda.

Development of Monoclonal Antibodies Against Cry1Ac/ Ab Protein for Designing of Sandwich ELISA to Detect BT Toxin from Cotton Seeds and Leaves

Order of Publishing in Issue: 
2
Volume :12
Issue :3
July, 2018 - September, 2018
Page No: 
218-229
Authors: 
Seetharavamma Gnagadasu[1&2], Jayant K Bhanushali[1], Usha Kiranmayi Mangamuri[3], K. R. S. Sambasiva Rao[1], Sudhakar Poda[1*]
Address: 
[1]Amar Immunodiagnostics Pvt, Ltd, Plot No 242/1, Road No 18, Jubilee Hills, Hyderabad-500033, Telangana, India
Address: 
[2]Department of Botany and Microbiology and Department of Biotechnology, Acharya Nagarjuna University, Guntur-522510, Andhra Pradesh, India
Address: 
[3]Department of Biotechnology, Acharya Nagarjuna University, Guntur-522510, Andhra Pradesh, India

The design of the study is to develop monoclonal antibodies against Cry1Ac/Ab protein for designing os sandwich ELISA (hybridoma technology). Hybridoma technology was invented by Cesar Milstein and Georges J.F Kohler in the year 1975 and is an unique method used to produce identical antibodies in maximum quantities. Monoclonal antibodies were developed by immunization of Balb/C mice with Cry1Ac/Ab Protein. Titer values of mice tail bleeds were checked and the best mice with higher titer value was used for fusion.

Keywords: 
Bacillus thuringiensis, Hybridoma Technology, Cry1Ac/Ab, HAT medium, Monoclonal antibodies, Iso-typing, genetically modified crops and Immunization.

 
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