Assessment of free radical scavenging activities and antioxidative potential of the tuber extracts of Stemona tuberosa Lour.

Order of Publishing in Issue: 
12
Volume :14
Issue :3
July, 2020 - September, 2020
Page No: 
347-358
Authors: 
C. Lalmuansangi, Marina Lalremruati and Zothansiama*
Address: 
Department of Zoology, Mizoram University, Aizawl-796 004, India
Email-ID: 
zothans@gmail.com

This study evaluates the phytochemical constituents, free radical scavenging activities and antioxidative potential of the tuber extracts of Stemona tuberosa (ST). The preliminary phytochemical screening revealed the presence of alkaloids, cardiac glycosides, saponins, steroids, tannins and terpenoids from various solvent extracts of ST. The methanolic extract of ST showed the highest phenolic (715.20 ± 2.42 mg GAE/g dry extract) and flavonoid (3864.25 ± 7.54 mg quercetin/g dry extract) contents. S.

Keywords: 
Stemona tuberosa, Free radicals, Antioxidants, Lipid peroxidation.

Morphological and biochemical characterization of fluorescent Pseudomonads from groundnut rhizosphere

Order of Publishing in Issue: 
11
Volume :14
Issue :3
July, 2020 - September, 2020
Page No: 
340-346
Authors: 
Padma Gurucharan Jogi[1], L.Nirmala Jyothi[1], K. Vijay Krishna Kumar[2], P. Osman Basha[1] and E.C. Surendranatha Reddy[1,*]
Address: 
[1]Department of Genetics and Genomics, Yogi Vemana University, YSR Kadapa, A.P., India
Address: 
[2]Acharya N G Ranga Agricultural University, Regional Agricultural Research Station, Maruteru, Andhra Pradesh, India

Groundnut is an important grain legume and oil seed crop grown all over the world. Fluorescent Pseudomonads are beneficial bacteria that inhabit the root zone of plants and increase the growth of plants by a wide variety of mechanisms. In our studies,, we have assessed the morphological and biochemical characteristics of 24 fluorescent pseudomonads isolated from groundnut rhizosphere collected from Rayalaseema region of Andhra Pradesh. All the Pseudomonas fluorescens (Pf) strains showed development of fluorescent pigments under UV light.

Keywords: 
Groundnut, rhizosphere, Pseudomonas fluorescens, Plant Growth Promotion.

Chrysin pretreatment improves mitochondrial enzymes and angiotensin converting enzymes in L-NAME induced hypertensive rats

Order of Publishing in Issue: 
10
Volume :14
Issue :3
July, 2020 - September, 2020
Page No: 
327-339
Authors: 
Veerappan Ramanathan[1], GV Swarnalatha[2], B. Manimegalai[1], A. Dominic Amalraj[1], Senthilkumar Rajagopal[2,*]
Address: 
[1]Department of Biochemistry, Enathi Rajappa Arts and Science College, Pattukkottai, TN, India
Address: 
[2]Department of Biochemistry, Rayalaseema University, Kurnool, AP, India
Email-ID: 
senthilanal@yahoo.com

Hypertension is one among the important factors that causes cardiovascular disorders. Nùnitro- L-arginine methyl ester (L-NAME) induces hypertension by blocking nitric oxide (NO) synthesis. Aim of present study was to investigate the effects of chrysin is one of major flavnoids, on L-NAME-induced hypertensive rats. Induces hypertension in adult male wistar rats weighing 180-220 g by oral treated of L-NAME (40 mg/kg/ day) dissolved in drinking water daily for 8 weeks. Experimental rats were oral treated with chrysin (25 mg/kg b.w).

Keywords: 
Angiotensin II, Chrysin, hydroxyproline, β - ketoglutarate dehydrogenase, nitric oxide, renin-angiotensin system.

Production and characterization of a haloalkaline pectinase from Halomonas pantellerinsis strain SSL8 isolated from Sambhar lake, Rajasthan

Order of Publishing in Issue: 
9
Volume :14
Issue :3
July, 2020 - September, 2020
Page No: 
319-326
Authors: 
Makarand N. Cherekar and Anupama P. Pathak*
Address: 
School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded-431606

Haloalkaliphilic bacterium producing a pectinase was isolated from the Sambhar soda lake, Rajasthan, India. Chemical composition of water sample was analyzed. Pectinase production was studied in submerged fermentation, an appropriate medium for the growth and production was orange peel powder. The bacterium was gram-negative and identified as Halomonas pantellerinsis strain SSL8 using biochemical tests and 16S rRNA sequencing. It was able to grow and produced pectinase that was stable and active at high pH, temperature and high NaCl concentration.

Keywords: 
Haloalkaliphilic, Halomonas pantellerinsis, Sambhar lake, orange peel powder, Pectinase

A simple and novel sample preparation approach for effective characterization of antibody low molecular weight impurities by CE-SDS method

Order of Publishing in Issue: 
8
Volume :14
Issue :3
July, 2020 - September, 2020
Page No: 
311-318
Authors: 
Bala Reddy Bheemareddy[1], Pradeep Iyer[1], Kranthi Vemparala[1], Vijaya R. Dirisala[2,*]
Address: 
[1]R&D Division, Hetero Biopharma Limited, Jadcherla, Mahaboob Nagar, Telangana, India – 509301
Address: 
[2]Department of Biotechnology, Vignan’s University, Guntur, Andhra Pradesh, India-522213
Email-ID: 
drdirisala@gmail.com

Product and process-related impurities of biopharmaceuticals have serious implications on product safety and efficacy in clinical use. Low molecular weight (LMW) impurities are generated during process and stability studies are routinely analyzed using non-reducing capillary electrophoresis with SDS during different stages of product development and release. The current sample processing methodology with heat denaturation is known to induce fragmentation and interfere with the LMW impurity analysis.

Keywords: 
Sample buffer, capillary electrophoresis, anti-CD20 monoclonal antibody, pH, Low molecular weight impurities.

Anticancer potential of D-limonene and hispolon against colon cancer cell lines

Order of Publishing in Issue: 
7
Volume :14
Issue :3
July, 2020 - September, 2020
Page No: 
302-310
Authors: 
K. Chandra Sekhar[1, 2], A. Rajanikanth[1], Md. Nazneen Bobby[2] and Jagadeeswara Reddy Kanala[1,*]
Address: 
[1]Sugen Life Sciences Pvt. Ltd, Tirupati – 517505, A.P., India
Address: 
[2]Department of Biotechnology, Vignan University, Vadlamudi, Guntur-522213, AP, India
Email-ID: 
kjreddy32@gmail.com

Natural products and associated combination therapy have gained prominent role in decreasing the adverse effects of synthetic drugs engaged in the severity of colon cancer. DLimonene a dietary monoterpene and hispolon a bioactive polyphenol proven to be anticancer agents independently against several cancers. The current study is designed to examine complementary anticancer effect of D-limonenehispolonconcoction in COLO-205 and HCT-116 cell lines.

Keywords: 
Colorectal cancer, D-limonene, Hispolon.

Green synthesis of silver nanoparticles from fractionated Annona reticulata leaf extract in different solvents and analysis of its antioxidant and antibacterial activity

Order of Publishing in Issue: 
6
Volume :14
Issue :3
July, 2020 - September, 2020
Page No: 
289-301
Authors: 
Sugunakar.Y.J, Chandramati Shankar P
Address: 
Department of Biotechnology, Yogi Vemana University, Kadapa, 516005 Andhra Pradesh, INDIA.
Email-ID: 
pchandra20@gmail.com

Silver nanoparticles were synthesized from fractionated leaf extract in Hexane, Chloroform and Water. Synthesis of AgNPs was confirmed by change in color of leaf extract solution, followed by confirming of reduction of silver ions in the leaf extract by UV-Visible spectroscopy. The Surface Plasmon resonance (SPR) peak was observed from 400 to 450nm. The biosynthesized AgNPs were characterized by dynamic light scattering measurement (DLS), Zeta potential and Transmission electron microscopy (TEM).

Keywords: 
AgNPs, Biosynthesis, Crystalline, Phytochemicals,Zeta potential.

Enhanced L-lysine production through chemical mutagenesis in Corynebacterium glutamicum MTCC 25069

Order of Publishing in Issue: 
5
Volume :14
Issue :3
July, 2020 - September, 2020
Page No: 
279-288
Authors: 
Ramesh Malothu
Address: 
School of Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kakinada - 533003, A.P, India

Lysine is one of the most commercially available amino acid, which is chiefly used as a feed additive, human medicine and as a dietary supplement. In the present day, Corynebacterium glutamicum is the most popularly known microorganism for the industrial biosynthesis of lysine. The purpose of this study was to improve the yield of lysine production by chemical mutagenesis. In this study the culture conditions were optimized and the maximum growth was observed at 37p C, pH-7.4 for 72 hours.

Keywords: 
L-lysine, Corynebacterium glutamicum, N Ethyl N NitrosoUrea (ENU), mutagenesis, auxotrophs, seed culture medium.

Generation of gamma irradiated mutagenized population in Solanum lycopersicum CV. Arka Vikas

Order of Publishing in Issue: 
4
Volume :14
Issue :3
July, 2020 - September, 2020
Page No: 
271-278
Authors: 
Pottipadu John Elia Prashanth, Nambi Rajesh, Jogi Gurucharan Padma, E.C. Surendranatha Reddy and Pinjari Osman Basha*
Address: 
Department of Genetics and Genomics, Yogi Vemana University, Kadapa

Natural and induced mutants are the primary resource for understanding the functions of the genes. The study of lethal dose (LD) and development of 300Gy, 400Gy, and 500Gy gamma irradiation population in Solanum lycopersicum L. cv. Arka Vikas was carried out in the present investigation. The highest germination percentage was recorded in control (98.53%). The germination percentage reduction, seedling height decline, and decreased pollen fertility in M1 generation was noted with the increasing gamma radiation dose.

Keywords: 
Solanum lycopersicum L., gamma irradiation, mutagenesis, morphological variations.

Formulation of pullulan/plasticizer blended films for their physical and biodegradability studies

Order of Publishing in Issue: 
3
Volume :14
Issue :3
July, 2020 - September, 2020
Page No: 
263-270
Authors: 
V S Rama Krishna Ganduri[1,*], Usha Kiranmayi M[2], K.R.S.Sambasiva Rao[3], Sudhakar Poda[3]
Address: 
[1]Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram- 522502, A.P., India
Address: 
[2]Department of Microbiology and Botany, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur- 522510, A.P., India
Address: 
[3]Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur- 522510, A.P., India

The present investigation utilizes the ecoefficient pullulan polysaccharide as film-forming biopolymer. Pullulan-based edible films offer good physical, thermal and mechanical properties which enable them to use in shelf-life preservation of fresh produce. Blends of other film-forming polysaccharides, plasticizers and an antioxidant with pullulan (Pu) solution were prepared in order to determine physical and optical parameters of those films. The morphological and biodegradable studies were attempted to identify the changes on the films’ surfaces.

Keywords: 
Pullulan, Plasticizers, antioxidant, edible film, biodegradable

 
To download full text articles and issues in PDF at no cost.All you have to do is fill out a brief registration form. It's easy and it's free. At any time, you may request removal of your contact information from our database.
Click here to Register | Click here if you have already registered

2018 ABAP Awards

Invitation for Nominations to 2019 ABAP Awards
The Association of Biotechnology and Pharmacy invites nominations for the following awards from the eligible talented people with proven track record and achievements on or before August 31, 2019 in the prescribed format.

DOWNLOAD CIRCULAR | APPLICATION FORM

AWARDS

Upcoming 0 events

  • No upcoming events available

User login