Production, Characterization and Evaluation of thrombolytic activity of Staphylokinase of Staphylococcus hominis

Order of Publishing in Issue: 
7
Volume :11
Issue :4
October, 2017 - December, 2017
Page No: 
389-395
Authors: 
C P Chandrappa[1]*, Megha Singh[1], N Chandrasekar[2] and M Govindappa[3]
Address: 
[1]Department of Biotechnology, Shridevi Institute of Engineering and Technology, Sira Road, Tumkur- 572106, Karnataka, India
Address: 
[2]Department of Chemistry, Shridevi Institute of Engineering and Technology, Sira Road, Tumkur-572106, Karnataka, India
Address: 
[3]Department of Biotechnology, Dayananda Sagar College of Engineering, Bengaluru, India

Staphylokinase isolated from Staphylococcus hominis has therapeutic function to dissolve the blood clots. Staphylococcus hominis was isolated from curd. Staphylococcus spp was confirmed by morphological, biochemical and molecular techniques such as 16s rDNA sequencing. Satoh’s medium was used for the production Staphylokinase. Cells were separated from culture broth by centrifugation and the supernatant fluid was added to 3 volume of acetone.

Keywords: 
Staphylokinase, Staphylococcus hominis, SDS-PAGE, Caseinolytic, Blood clot.

An updated review on Phytochemical and Pharmacological propeties of Piper sarmentosum

Order of Publishing in Issue: 
2
Volume :11
Issue :4
October, 2017 - December, 2017
Page No: 
345-356
Authors: 
Muhammad Shahzad Aslam*[1], Muhammad Syarhabil Ahmad[1], Muhammad Ayaz Ahmad[2] and Muhammad Akhlaq[3]
Address: 
[1]School of Bioprocess Engineering, Universiti Malaysia Perlis (UniMAP), Kompleks Pusat Pengajian Jejawi 3 (KPPJ3), Kawasan Perindustrian Jejawi, 02600, Arau, Perlis, Malaysia.
Address: 
[2]Physics Department, Faculty of Science, P.O.Box 741, University of Tabuk, 71491, Saudi Arabia.
Address: 
[3]Department of Pharmacy, Abasyn University, Islamabad Campus (AUIC) Park Road, Islamabad, Pakistan.

Piper sarmentosum is a well-known medicinal plant of Southeast Asia consist of many medicinal properties such as Anti-microbial, antifungal, antimalarial, antituberculosis, antimycobacterial, anticarcinogenic, antioxidant, antinociceptive, anti-inflammatory, antiamoebic, neuromuscular blocking activity, anti-obesity, antiangiogenesis, wound healing, cardiovascular activity, anti-hypertensive, antiulcer and Anticoccidial activity.

Keywords: 
Piper sarmentosum; Phytochemistry, Pharmacology, Alkaloid amide, Southeast Asia.

Plant Regeneration of a Spring Vegetable -Asparagus officinalis L. Through Somatic Embryogenesis

Order of Publishing in Issue: 
1
Volume :11
Issue :4
October, 2017 - December, 2017
Page No: 
334-344
Authors: 
Mustafa Abul Kalam Azad* and Muhammad Nurul Amin
Address: 
Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh
Email-ID: 
drmakazad@gmail.com

A plant regeneration protocol was established from hypocotyl explants of in vitro grown seedlings of Asparagus officinalis and in vitro-proliferated shoots, respectively through either somatic embryogenesis or embryogenic callus. Types of auxins and cytokinins play an important role for producing somatic embryos.

Keywords: 
Embryos, Growth regulators, Hypocotyl, Embryogenic callus, Coconut water.

News Item

Order of Publishing in Issue: 
13
Volume :11
Issue :3
July, 2017 - September, 2017
Page No: 
i-v
Authors: 
Editor
Email-ID: 
editor@abap.co.in
Keywords: 
News item

Computational Biology as an aid in Deciphering vital insights on Plant Pathogens and their Interaction with hosts

Order of Publishing in Issue: 
12
Volume :11
Issue :3
July, 2017 - September, 2017
Page No: 
322-333
Authors: 
Anurag Kashyap*, Borsha Rani Baruah and PD Nath
Address: 
Department of Plant Pathology, Assam Agricultural University, Jorhat, 785001, Assam, India

Computational biology or bioinformatics has its roots in the dawn of genomic revolution when research activities were started to sequence the genome of various organisms. Refinement in sequencing technologies and potential of genomic research resulted in meteoric growth of biological information such as sequences of DNA, RNA and protein requiring databases for efficient storage, management and retrieval of the biological information. Also, computational algorithms for analysis of these colossal data became a vital aspect of biological sciences.

Keywords: 
Bioinformatics, Databases, Genome sequencing, Plant pathology research.

Identification of Quantitative Trait Loci for Panicle Associated Traits in Recombinant Inbred Line Population Derived from Japonica X Indica sub-species in Rice (Oryza sativa L.)

Order of Publishing in Issue: 
11
Volume :11
Issue :3
July, 2017 - September, 2017
Page No: 
316-321
Authors: 
Prabha R. Chaudhari*, D.K.Mishra, Suchita S. Xalxo , Ritu R. Saxena, Laxmi Singh and S.B. Verulkar
Address: 
Department of Genetics & Plant Breeding, IGKVV, Raipur- 492012 (CG), India
Email-ID: 
chau.prabha@gmail.com

Rice recombinant inbred lines (RILs) of Japonica x Indica cross were grown in irrigated conditions for identification of quantitative trait loci (QTLs) for panicle associated traits. One hundred twenty one RIL lines were evaluated in the field environment with two replications. A total of one hundred twenty two SSR markers were used for polymorphism out of forty markers that showed parental polymorphism. Forty SSR markers were used to construct the genetic linkage map by employing single marker analysis.

Keywords: 
Rice, Single marker analysis, Panicle traits, QTL identification.

Chemometric-assisted UV Spectrophotometric and RPHPLC Methods for the Simultaneous Determination of Caffeine and Sodium Benzoate in Synthetic Mixture

Order of Publishing in Issue: 
10
Volume :11
Issue :3
July, 2017 - September, 2017
Page No: 
309-315
Authors: 
Akshay R. Pahade[1], Santosh V. Gandhi[1]* and Shreeyash R. Tapale[1]
Address: 
[1]AISSMS College of Pharmacy, Kennedy Road, Pune- 411001, Maharashtra, India.

In present work, chemometric-assisted UV spectrophotometric and RP-HPLC methods weredeveloped for the simultaneous estimation of caffeine (CAF) and Sodium benzoate (SB) in their combined pharmaceutical dosage form. The two chemometric methods i.e. principle component regression (PCR) and partial least square regression (PLS) were successfully applied to quantify each drug in mixture using UV absorption spectra in range of 220 – 280 nm at Δλ of 1 nm.

Keywords: 
Chemometric, PCR, PLS, HPLC, Caffeine, Sodium benzoate.

Optimization for Enhanced Production of Antibacterial Metabolites by Marine Actinomycetes Kocuria sp. strain rsk4

Order of Publishing in Issue: 
9
Volume :11
Issue :3
July, 2017 - September, 2017
Page No: 
300-308
Authors: 
Ravi Ranjan Kumar[1]* and Vasantba J Jadeja[2]
Address: 
[1]Department of Biotechnology, [2]Department of Microbiology Shree M. & N. Virani Science College, Kalawad Road, Rajkot 360005, Gujarat, India
Email-ID: 
raviranjan@vsc.edu.in

A new halophilic actinomycetes strain was characterized and on the basis of physiological and 16S rDNA sequence, it was designated as Kocuria sp. strain rsk4. It was able to produce antibacterial metabolites and shows broad spectrum antibacterial activity against several gram-positive and gram-negative bacteria.

Keywords: 
Actinomycetes, Antibacterial metabolites, Culture condition, Kocuria, Optimization.

Cloud Computing and its Need in Life Science’s R & D

Order of Publishing in Issue: 
8
Volume :11
Issue :3
July, 2017 - September, 2017
Page No: 
294-299
Authors: 
L. N. Chavali[1], N. Lakshmi Prashanthi[2], K. Sujatha[3] and P. B. Kavi Kishor[1]*
Address: 
[1]Department of Genetics, Osmania University, Hyderabad - 500 007, India
Address: 
[2]GITAM Institute of Management, GITAM University, Rushikonda, Visakhapatnam 530 045, Andhra Pradesh, India
Address: 
[3]Department of Chemistry, KTR Women’s College, Gudivada- 521 301, Andhra Pradesh, India
Email-ID: 
pbkavi@yahoo.com

The cloud computing is driven by factors such as aging of the current information technology (IT) infrastructure and changes in IT landscape. Virtualization and cloud computing have transformed the way the IT services are delivered to customers at a lower cost. The research and development (R&D) in life sciences is on cusp of change. The new opportunities and challenges must focus on renewing the existing systems and processes for efficacy and adopting advancements in new technologies to gain value.

Keywords: 
Nil

Stability Indicating Assay Method for the Determination of Medroxy Progestrone Aceate in Bulk Drug and Formulation by HPTLC

Order of Publishing in Issue: 
7
Volume :11
Issue :3
July, 2017 - September, 2017
Page No: 
286-293
Authors: 
Girish Pai Kulyadi[2], Vasanthraju[1], Prashanth Musmade[1] and Muddukrishna Badamane Sathyanarayana[1]*
Address: 
[1]Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka-576104.
Address: 
[2]Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka-576104.

A sensitive, accurate, precise and stability indicating high-performance thin layer chromatographic assay method for analysis of Medroxyprogestrone acetate (MPA) in both bulk drug and formulation was developed and validated. The method employed HTLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of Toluene: ethyl acetate: ammonia solution (80:20:0.1 %v/ v). This system was found to give compact spots for MPA (Rf value 0.32±0.02).

Keywords: 
Nil

 
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