News Item

Order of Publishing in Issue: 
11
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
i-vii
Authors: 
Editor
Email-ID: 
editor@abap.co.in

News Item

Keywords: 
Nil

Modelling and Optimization of L-Asparaginase Production from Bacillus stratosphericus

Order of Publishing in Issue: 
10
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
390-405
Authors: 
Madhuri Pola[1], Chandrasai Potla Durthi[1], Satish Babu Rajulapati[1]*, Rajeswara Reddy Erva[2]
Address: 
[1]National Institute of Technology, Warangal
Address: 
[2]National Institute of Technology, Andhra Pradesh

L-Asparaginase has gained importance as one of the most important medications used to treat Acute Lymphocytic Leukemia (ALL). The goal of the current study is to maximize the LAsparaginase activity besides minimizing the LGlutaminase activity. Shorter half-life and side effects remain the drawbacks of the commercially available L-Asparaginase drugs. To overcome the drawbacks, L-Asparaginase has been isolated from endophytes of Ocimum tenuiflorum.

Keywords: 
L-Asparaginase, ALL, endophytes, OFAT, Plackett-Burman method, RSM.

Evaluation of Factors Affecting L-asparaginase Activity using Experimental Design

Order of Publishing in Issue: 
9
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
377-389
Authors: 
Rania A. Zaki, Mona S. Shafei, Heba A. El Refai *, Abeer A. El-Hadi and Hanan Mostafa
Address: 
Chemistry of Natural and Microbial Products Department, National Research Center, (ID:60014618), Dokki, Giza, Egypt
Email-ID: 
dr.heba_ar@yahoo.com

The present study aims for the purification and characterization of L-asparaginase produced by Fusarium solani. Microbial L-Asparaginase has attracted considerable attention, owing to the cost effective and eco-friendly nature. The most common use of asparaginases is as a processing aid in the manufacture of food. L-asparaginase used to reduce the formation of acrylamide, a suspected carcinogen, in starchy food products such as baked or fried snacks and biscuits.

Keywords: 
Fusarium solani -L-Asparaginase - Solid State Fermentation- partially purified enzyme- sequential statistical strategy.

Molecular Phylogenetic Analysis of Indian Apple Snail

Order of Publishing in Issue: 
8
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
371-376
Authors: 
Silpi Sarkar and S. Krupanidhi
Address: 
Department of Biotechnology, VFSTR Vadlamudi, 522213 A P, India

Apple snails (Ampullariidae) belong to a diversified freshwater family which occurs in pantropical habitats. The Indian apple snail, Pila globosa and other operculate snails are grouped in Architaenioglossa which also includes Viviparidae and Cyclophoridae. In our study, a concise relationship of Ampullariidae with Viviparidae and Cyclophoridae has been elucidated by using partial nucleotide sequence of cytochrome b gene.

Keywords: 
Pila globosa, Morphometry, Cytochrome b gene, RAxML , MEGA-v5.2

Protein Characterization at Atomic Level: A Novel Approach for Sequence Analysis

Order of Publishing in Issue: 
7
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
367-370
Authors: 
Parul Johri[1]*, Mala Trivedi[1], Drishti Srivastava[1], Aman Kumar Singh[1] and Mohammed Haris Siddiqui[2]
Address: 
[1]Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Malhaur, Gomti Nagar Extension, Lucknow, Uttar Pradesh, India
Address: 
[2]Department of Bioengineering, Integral University Lucknow, Uttar Pradesh, India
Email-ID: 
pjohri@lko.amity.edu

Carbon being the most omnipresent element in all the organic compounds is of great importance with regards to its structure and function. The fundamental structure of protein is composed of amino acids arranged in linear chain and folded to a globular form. All twenty amino acids consist of combination of only five different atoms that are Carbon, Nitrogen, Oxygen, Hydrogen and Sulphur. Depending on the property of their side chain the amino acids are classified as hydrophobic and hydrophilic.

Keywords: 
Carbon, E. Coli, dynamic programming, proteins.

Evaluation of Antimicrobial Activity of Emblica officinalis against Skin Associated Microbial Strains

Order of Publishing in Issue: 
6
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
355-366
Authors: 
Lovey Sharma[1]* and Ram Kumar Pundir[1]
Address: 
[1]Department of Biotechnology, Ambala College of Engineering and Applied Research, Devsthali (near Mithapur), P.O. Sambhalkha-133101, Ambala (Haryana)-India

The present study was undertaken to access antimicrobial activity of fruit extracts of Amla (Emblica officinalis) against skin associated microorganisms. The antimicrobial activity of five different solvents viz. methanol, ethanol, distilled water, chloroform and petroleum ether against Gram positive, Gram negative bacteria and yeast namely Propionibacterium acne, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans was assessed by using agar well diffusion method.

Keywords: 
Antimicrobial activity, Emblica officinalis fruit, GC-MS analysis, Phytochemicals, Skin disease.

Insilico Studies of FOR20 - A Centrosomal Protein

Order of Publishing in Issue: 
5
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
349-354
Authors: 
A. RanganadhaReddy[1]*, N. MadhanSai[1], S. Krupanidhi[1], P. Sudhakar[2], and T.C. Venkateswarulu[1]
Address: 
[1*]Department of Biotechnology, VFSTR (Deemed to be University) Guntur - 522213, India
Address: 
[2]Department of Biotechnology, Acharya Nagarjuna University Guntur - 522 510, India
Email-ID: 
rangaaluri@gmail.com

There is enough scientific evidence on microtubule playing important role in various functions, one such is chromosomal segregation, which made them as primary targets in the cancer treatment. Centrosomal proteins plays vital role in maintaining microtubule dynamics that having significant effect on various functions involving microtubule such as chromosomal segregation, intracellular transport and cell polarity.

Keywords: 
FOR20 protein, Centrosomal protein, SWISS MODEL, PROCHECK.

An in silico Structural study on Bacterial Sulfite Reductase

Order of Publishing in Issue: 
4
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
342-348
Authors: 
Rajeswara Reddy Erva[1]*, Rinku.P.Varghese[2], A.L.Prasanna[2], G.Madhunika[2], K.S.Ravi Teja[2], V.S.Santosh[2], Satish Babu Rajulapati[3]
Address: 
[1]Department of Biotechnology, National Institute of Technology Andhra Pradesh, Tadepalligudem, Andhra Pradesh, India.
Address: 
[2]School of Biotechnology, VFSTR (Deemed to be University), Vadlamudi, Andhra Pradesh, India.
Address: 
[3]Department of Biotechnology, National Institute of Technology Warangal, Warangal, Telangana, India.

Sulfur is amongst the most profuse elements on the globe and a vital component for life maintenance. Sulfite reductase plays a key role in sulfur pathway. It converts sulfite to sulfide. Reductase enzyme is present in sulfur reducing bacteria. Three organisms from Desulfovibrio species namely, D. alaskensis, D. vulgaris, Desulfovibrionaceae are used in this study. The computational analysis has been done out on the sulfite reductase enzyme form the above listed sulfur reducing bacteria using in silico tools.

Keywords: 
Sulfite Reductase, Sulfur reducing bacteria, Structure analysis, Homology modeling and structural validation.

Purification of Foot and Mouth Disease Virus Nonstructural Protein 3ABC from Vaccine in-process Samples and their Characterization

Order of Publishing in Issue: 
3
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
334-341
Authors: 
Anil Kumar Jangam[1], Sridevi V Nimmagadda[1] , Premalatha Dasari[†] and Rajendra Lingala[1]*
Address: 
[1]Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad- 500032, Telangana, India.
Address: 
[†]Department of Biotechnology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad- 500085, Telangana, India.

Foot and mouth disease is a highly contagious disease that affects the cloven hoofed animals which includes livestock and can impact on nation’s economy. Adjuvanted inactivated whole virus (140s) is used as vaccine to protect animals and control the disease. The vaccine production process is well established by many commercial manufacturers by culturing the virus on BHK cell line.

Keywords: 
FMDV, NSP, Chromatography, ELISA.

Development of Murine Monoclonal Antibodies, Characterization and Quantification of Diphtheria Toxoid in Vaccine Batches

Order of Publishing in Issue: 
2
Volume :12
Issue :4
October, 2018 - December, 2018
Page No: 
318-333
Authors: 
Praveen Alagangula[1,2], Sridevi V Nimmagadda[1], Vidyasagar Pitta[1], Dipankar Das, Ralla Kumar[1], Shukra M Aavula[1], Mohammed Furman Ali[1], Premalatha Dasari[1], Rajendra Lingala[1]*
Address: 
[1]Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad- 500032, Telangana, India.
Address: 
[2]Department of Biotechnology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad- 500085, Telangana, India.

Diphtheria is caused by Cornybacteria diphtheria and it is one of the contagious diseases with a mortality rate of 5% to 10% worldwide. Though the mass immunization of diphtheria vaccine reduces the mortality, but the immediate effective treatment for this disease involves the administration of anti-diphtheria polyclonal antibodies or diphtheria antitoxin (DAT) produced from Equines. However the availability of antidiphtheria polyclonal antibody is very limited due to the less number of manufacturers.

Keywords: 
Monoclonal antibodies, Diphtheria toxin, ELISA, In vitro neutralization.

 
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