Callus Induction and Organogenesis in Sesamum indicum L. CV. E 8

Order of Publishing in Issue: 
10
Volume :5
Issue :4
October, 2011
Page No: 
1462-1468
Authors: 
Srinath Rao* and Havgeppa N. Honnale
Address: 
Plant Tissue Culture and Genetic Engineering Laboratory Department of Post-Graduate Studies and Research in Botany, Gulbarga University Gulbarga - 585 106, Karnataka, India

Abstract:
Hypocotyl and cotyledon explants excised from 5-7 days old in vitro grown seedlings of sesame were inoculated for callus induction and direct regeneration on MS medium supplemented with different concentrations(1-4mg/l) and combinations of auxins viz., NAA, 2, 4-D, 2, 4, 5-T, IAA and IBA. Hypocotyl and cotyledon explants were found suitable for induction of callus on MS medium supplemented with NAA at 2.0mg/l. Hypocotyl was found to be a better explant for the induction of maximum callus. Cytokinins like BAP, Kn and TDZ were used for regeneration from callus. Regeneration from callus was not obtained on any of the cytokinins supplemented media. Direct regeneration from hypocotyl (40%) explant was obtained on MS medium supplemented with 1.0 mg/l Kn compared to other cytokinins. The plant-lets were subsequently rooted on MS medium supplemented with IBA (3.0 mg/l).

Keywords: 
Sesamum indicum, Callus induction, organogenesis, NAA, Kn, IBA
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