Cadmium affects Phosphatase activity leading to Reduced Power assay of Barley (Hordeum vulgare L.)

Order of Publishing in Issue: 
5
Volume :8
Issue :1
January, 2014
Page No: 
38-44
Authors: 
Udita Gubrelay[1]*, Rajneesh K. Agnihotri[2], Gurpreet Singh[2], Ravinder Kaur[1] and Rajendra Sharma[2]
Address: 
[1]Department of Biochemistry, School of Life Sciences, Khandari Campus, Dr. B.R. Ambedkar University, Agra – 282002, India
Address: 
[2]Department of Botany, School of Life Sciences, Khandari Campus, Dr. B.R. Ambedkar University, Agra – 282002, India

Abstract
Heavy metal stress is an important factor which may influence plants’ growth and metabolism. The present study investigates the effects of cadmium (soil treated twice/ week with 10, 20 and 30 mM) on the specific activities of phosphatases which might lead to reducing power assay in Barley (Hordeum vulgare L, var 2052) seedling. A significant decrease in the redox potential of shoot compared to root was observed at the similar concentration of cadmium. A similar trend on leaves were also noted. Acid and alkaline phosphatase specific activities were significantly higher in roots than in shoot at all the three concentration of cadmium i.e. 10, 20 and 30mM, compared to controls. The above mentioned changes were more pronounced at 30mM concentration of cadmium than two other concentration. These results lead us to suggest that increased cadmium concentration in soil might lead to an adverse effects on plant growth and phosphatase activities.

Keywords: 
Acid phosphatase, Alkaline phosphatase, Cadmium induced stress, Hordeum vulgare, Reducing power assay.
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