Biochemical Studies During Sequential stages of Root and Shoot Differentiation in Callus cultures of Cardiospermum halicacabum L.

Order of Publishing in Issue: 
Volume :9
Issue :4
November, 2015
Page No: 
Ashwani Kumar[1, 2*], S.C. Goyal[1], Pooja[1], Charu Lata[2] and Jagdish Parshad[3]
[1]Department of Botany & Plant Physiology, CCS HAU, Hisar – 125004, Haryana, India.
[2]ICAR-Central Soil Salinity Research Institute, Karnal – 132001, Haryana, India.
[2]Department of Microbiology, CCS HAU, Hisar – 125004, Haryana, India.

Present investigation was carried out on Cardiospermum halicacabum L. (Ballon Vine) - an important medicinal plant. Leaf explants were found to be the best for induction and growth of callus. Depending on fresh and dry weight of callus, high growth value callus was obtained on MS medium supplemented with 2, 4-D (1.0 mg/l) in combination with Kn (1.0 mg/l). This callus was selected for further studies of root and shoot organogenesis as well as biochemical studies. Regeneration of roots (15 days) from callus was observed on MS medium + BAP (0.5 mg/l) + NAA (0.3 mg/l) while shoots (16 days) on MS medium + BAP (0.5 mg/l) + NAA (0.1 mg/l). Starch content and reducing sugars were high in control or undifferentiated callus, which further increases significantly in root and shoot differentiating cultures. Contents of total soluble sugars, total soluble proteins and total phenols were lower in the control callus which increases in the root and shoot differentiating cultures. The activities of enzymes i.e. a-amylase, acid protease and peroxidase increased up to appearance of green patches (8th day) and were reached at peak on 12th day, the day that coincide with the visual appearance of roots and shoot. Conversely, the acid invertase activity decreases till the appearance of roots and shoots.

Ballon vine, callus, metabolites, enzymes, differentiation.
Paper-9.pdf1.92 MB

To download full text articles and issues in PDF at no cost.All you have to do is fill out a brief registration form. It's easy and it's free. At any time, you may request removal of your contact information from our database.
Click here to Register | Click here if you have already registered

2018 ABAP Awards

Invitation for Nominations to 2019 ABAP Awards
The Association of Biotechnology and Pharmacy invites nominations for the following awards from the eligible talented people with proven track record and achievements on or before August 31, 2019 in the prescribed format.



Upcoming 0 events

  • No upcoming events available

User login