Bacillus Amyloliquefaciens: A new Keratinolytic Feather-degrading Bacteria

Order of Publishing in Issue: 
16
Volume :2
Issue :1
January, 2008 - March, 2008
Page No: 
170-177
Authors: 
M. Cortezi, [1]. E. M. Cilli [2], J Contiero*[1]
Address: 
[1] Department of Biochemistry and Microbiology, Biological Sciences Institute, São Paulo State University-Unesp, São Paulo, Brazil
Address: 
[2] Department of Biochemistry and Chemical Technology, Institute of Chemistry, São Paulo State University-Unesp, São Paulo, Brazil
Email-ID: 
jconti@rc.unesp.br

Abstract:Feather-degrading microorganisms were isolated from a poultry processing industry. The microorganism that presented high degradation of feathers was LM16, reaching 97.7% after 72 h of fermentation. Using morphological and biochemical tests [API 20 E (Bio-Merieux)], the strain was identified as Bacillus amyloliquefaciens. The keratinase production and cell growth were affected by culture conditions. The optimal conditions for enzyme production were 40ºC, 150 rpm, 1% of feather and pH 7.5, resulting in an activity of 187.5 U/mL. The results reveal that the release of amino acids, as evidenced through thin-layer chromatography, in the broth of the Bacillus amyloliquefacines culture can allow its use in animal feed  industries (meal feather).

Keywords: 
Bacillus amyloliquefaciens, feathers degradation, keratinase, keratin, fermentation,
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