Automated image analysis to observe pellet morphology in liquid cultures of filamentous fungi such as the basidiomycete Coprinopsis cinerea

Order of Publishing in Issue: 
2
Volume :3
Issue :3
March, 2010
Page No: 
241 - 253
Authors: 
Martin RŸhl and Ursula KŸes*
Address: 
Molecular Wood Biotechnology and Technical Mycology, BŸsgen-Institute, Georg-August-University Gšttingen, BŸsgenweg 2, 37077 Gšttingen, Germany
Email-ID: 
ukuees@gwdg.de

Abstract
In this study, an image analysis system forfungal pellets was developed using thecommercial software system analySIS¨ and theprotocol was evaluated in morphological studiesof pellet formation in submerged cultures of thebasidiomycete Coprinopsis cinerea. Pellets wereanalysed on large scale (60 to 130 pellets perimage, 225 to 400 pellets per culture).Morphologies of pellets were characterised bythe parameters grey value, pellet area, convexity,shape factor, sphericity and pellet diameter.Threshold values were defined for all parametersfor object filtering. By application of theparameter filter, aggregated hyphal fragmentspresent in larger amounts particularly in culturesgrown at higher temperature (370C) could beclearly distinguished in image analysis fromcompact pellets. At a lower growth temperature(250C), there was little background of loosehyphal material, fungal pellets were regularlyshaped and the pellets remained constant in shapeand size over a longer cultivation period.

Keywords: 
Automated image analysis, filamentous organism, pellet morphology, hyphal aggregates, Coprinopsis cinerea.
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