Antiproliferative and antiangiogenic effects of partially purified Luffa acutangula fruit extracts on human lung adenocarcinoma epithelial cell line (A-549)

Order of Publishing in Issue: 
7
Volume :3
Issue :4
October, 2009 - December, 2009
Page No: 
396-404
Authors: 
Boreddy Purushotham Reddy, R. Kannaiah Goud, S. Venkata Mohan*, P. N. Sarma
Address: 
Bioengineering and Environmental Centre, Indian Institute of Chemical Technology Hyderabad 500 607, India
Email-ID: 
vmohan_s@yahoo.com

Abstract : The present study was undertaken to evaluate the possibility of Luffa acutangula (cucurbitaceae family) fruit as a potential anticancer agent by examining antiproliferative and antiangiogenic activities. Fruit methanolic extract  showed significant antiproliferative activity (IC-50, 131.63±2.31 ug/ml) on human lung adenocarcinoma epithelial cell line (A-549). The extractwas partially purified by chromatography. Fraction F2-3 showed most potent antiproliferative activity (IC-50, 7.61±1.03 ug/ml)  and was further evaluated for antiangiogenic activity by evaluating vascular endothelial growth factor (VEGF), matrix metalloproteinases-2 (MMP-2) and matrix metalloproteinases-2 (MMP-9) as in-vitro, chick chorioallantoic membrane (CAM) as an in-vivo model for VEGF. VEGF and both MMP protein expressions were significantly inhibited in F2-3 treated A-549 cells compared to control cells (VEGF: 4.36±0.47 and 14±0.75 pg/ml, MMP-2: 10.17±1.3 and 20.28±1.68, MMP-9: 12.93±1.70 and 21.12±2.12 ng/ml, respectively). Egg chorioallantoic membrane treated with F2-3 fraction (10 ?g) showed clear avascular zones compared to phosphate buffered saline (PBS) treated eggs. In conclusion, ourdata provides a scientific proof for Luffa acutangula as a potential antitumor agent.

Keywords: 
Vascular endothelial growth factor (VEGF), Matrix metalloproteinases MMP), Extracellular matrix (ECM), Chick chorioallantoic membrane (CAM) model.
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