Antioxidant and Free Radical Scavenging Properties of Traditionally Used Three Indian Medicinal Plants

Order of Publishing in Issue: 
8
Volume :2
Issue :4
October, 2008
Page No: 
538 -547
Authors: 
Prateek Kumar Jain, V. Ravichandran and R. K. Agrawal*
Address: 
Pharmaceutical Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar (M.P) - 470 003, India

The antioxidant properties of methanol extract of three medicinal plants viz. Withaniasomnifera, Asparagus racemosus and Centella asiatica that probably involve free radicalmechanisms, were evaluated by the methods, namely the ABTS (2, 2’-azinobis-3-ethylbenzothiozoline-6-sulphonic acid) radical scavenging assay, Trolox equivalent antioxidant capacity assay (TEAC) , Oxygen radical absorbance capacity (ORAC) assay, DPPH free radical scavenging activity assay, Super oxide scavenging activity assay and Nitric oxide radicalscavenging activity assay. The methanol extract from Centella asiatica was most active in theABTS assay with an IC50 value of 185.18 ?g/mL followed by Withania somnifera with IC50 valueof 214.78 ?g/mL. The minimum activity showed by Asparagus racemosus with IC50 value of384.55 ?g/mL. The Trolox equivalent antioxidant capacity, DPPH (1, 1-diphenyl-2-picryl hydrazyl)and Superoxide radical scavenging activity of the methanolic extracts was in order of Withaniasomnifera > Centella asiatica > Asparagus racemosus. In the Oxygen radical absorbancecapacity assay the ORAC value of Withania somnifera was found maximum 443.5 ? molesTE#/ g and followed by Centella asiatica and Asparagus racemosus showed 211.5 ? molesTE#/ g and 31.3 ? moles TE#/ g respectively . The Nitric oxide radical activity was found inorder of Asparagus racemosus >Withania somnifera > Centella asiatica. The presentstudies suggest that all the tested plants have moderate to potent antioxidant activity.

Keywords: 
Antioxidant activity, Withania somnifera, Asparagus racemosus, Centella asiatica, Gallic acid.
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