Antibacterial Activity of Carica papaya against Methicillin resistant Staphylococcus epidermidis Isolated from Wards Surfaces of Kampala International University Teaching Hospital, Bushenyi, Uganda

Order of Publishing in Issue: 
5
Volume :13
Issue :4
October, 2019 - December, 2019
Page No: 
391-400
Authors: 
Abubakar Sunusi Adam*[1], Ibrahim Ntulume[1], Rasheed Adeyemo[1], Saheed Akinola[1], Ibrahim Jatau Abubakar[2], Adamu Almustapha Aleiro[1,3], Sarah Onkobah[1], Lisa Micheni[1], Alice Namatovu[1,4]
Address: 
[1]Department of Microbiology and Immunology, Faculty of Biomedical Sciences, Kampala International University Western Campus, Ishaka, Uganda
Address: 
[2]Division of Pharmacy, College of Health and Medicine, University of Tasmania, Hobart, Australia
Address: 
[3]Department of Biological Sciences, Faculty of Science, Kebbi State University of Science and Technology, Kebbi State, Nigeria
Address: 
[4]Department of Biotechnical and Diagnostic Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, P. O. Box 7062, Kampala, Uganda

This study aimed at determining the antibacterial activity of Carica papaya against Methicillin resistant Staphylococcus epidermidis isolated from doorknobs, bed rails, floors and walls of Surgical, Medical, Maternity, Pediatrics, Accident and Emergency, Semiprivate and Private wards of Kampala International University Teaching Hospital. The bacteria were isolated from the wards surfaces and identified using biochemical tests, Desferroxiomine and Fosfomycin antibiotics. Disc diffusion method was used to detect methicillin resistance in S. epidermidis using Cefoxitin (30 μg) disc. Fresh leaves and seeds of C. papaya was processed and extracted using standard methods. Antibacterial activities of the methanol, acetone and aqueous crude extracts were assayed using the agar well diffusion method. Phytochemical analysis, Minimum Inhibitory and Bactericidal concentration of the crude extracts were determined using broth dilution methods. Both C. papaya leaf and seed crude extract exhibited antibacterial activity against MRSE with MICs and MBCs ranges of 250 to 31.3mg/ml and 125 to 31.3 mg/mL for leaf and seed extracts respectively. This study concludes that C. papaya leaf and seed crude extracts were effective against Methicillin resistant S. epidermidis.

Keywords: 
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