16S rRNA based identification and phylogenetic analysis of a novel dextran producing Pediococcus pentosaceus isolated from north-east Indian microbial diversity

Order of Publishing in Issue: 
2
Volume :4
Issue :3
July, 2010
Page No: 
746-754
Authors: 
Seema Patel and Arun Goyal
Address: 
Department of Biotechnology Indian Institute of Technology Guwahati Guwahati- 781 039, Assam, India

Abstract :

Looking at the high application potential of dextrans in food, pharmaceutical and tissue engineering fields, a dextran producing strain of lactic acid bacterium was isolated from the sugarcane field soil sample from Assam, falling under north-east Indian microbial diversity. The full-length 16S rRNA gene of the isolate was amplified by Polymerase Chain Reaction (PCR) and sequenced by Sanger’s method. The sequence was aligned with the reference sequences in the GenBank of National Centre for Biotechnological Information (NCBI) and Ribosomal Database Project (RDP) using BLAST programme. Distance matrix based phylogenetic analysis resulted in well-resolved trees with nodes supported with high bootstrap pseudoreplicate scores. The lactic acid bacterium isolate was identified as Pediococcus pentosaceus (GenBank Accession Number EU569832). This identification revealed the dextran production attribute of Pediococcus genus of lactic acid bacteria for the first time ever, heralding further rigorous investigation on this aspect. This investigation also unravelled the abundance of industrially valuable microbial flora in the soil of north-east India.

Keywords: 
Pediococcus pentosaceus, dextran, 16S rRNA gene, phylogenetic analysis.
AttachmentSize
Paper-2.pdf153.13 KB

 
To download full text articles and issues in PDF at no cost.All you have to do is fill out a brief registration form. It's easy and it's free. At any time, you may request removal of your contact information from our database.
Click here to Register | Click here if you have already registered

2017 ABAP Awards

Invitation for Nominations to 2017 ABAP Awards
The Association of Biotechnology and Pharmacy invites nominations for the following awards from the eligible talented people with proven track record and achievements on or before September 10, 2017 in the prescribed format.

DOWNLOAD CIRCULAR | APPLICATION FORM

AWARDS

User login

Counter

  • Site Counter: 0
  • Unique Visitor: 0
  • Registered Users: 0
  • Blocked Users: 0
  • Your IP: 54.162.241.40
  • Since:
  • Visitors:
    • Today:
    • This week:
    • This month:
    • This year: